الفهرس 
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Abstract
The present study was conducted on 120 newly born calves (37 mixed breed calves, 66 native breed calves and 17 buffaloes). The age of these animals between one day to six months, they examined during the period from April 2014 to April 2016, these animals belong to some villages and farms of Assiut governorate.
The clinical findings of cryptosporidiosis in examined calves were showed mild to severe diarrhea and varying degree of dehydration. Some cases were feverish; the state of appetite was different according to the severity of illness. The feces were varied from pasty to watery in consistency, pale yellow, yellow or greenish in color and sometimes contained mucous and blood.
The result of conventional technique (MZN smears) in the present study cleared that animals were infested with cryptosporidium oocysts (they were spherical to ovoid in shape). The prevalence rate was15.83 % (20.89% in animals less than two months, 11.36% in calves’ from2-4months with no infection rate in calves from 4-6 months).
Male calves were more susceptible than females calves to infection of cryptosporidiosis {males 16.44% (12/73) – females 14.89% (7/47)}.
Higher infection rate of Cryptosporidium in calves was in non-hot months (22.95%) more than hot months (8.47%).
The molecular technique used for identification of Cryptosporidium infection in calves was nPCR. This technique was performed on 50 fecal samples and revealed that 28% (14 samples) were positive.
Results declared that PCR was more sensitive than MZN stain in detection of Cryptosporidium.