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العنوان
Isolation of Stenotrophomonas Species from Raw Milk and some Dairy Products /
المؤلف
Hanna, Mariana Ibrahim Labib.
هيئة الاعداد
باحث / ماريانا ابراهيم لبيب
مشرف / ايناس البرنس محمد
مناقش / نجاح محمد سعد
مناقش / عادل محمد مصطفي خولي
الموضوع
Milk - Microbiology.
تاريخ النشر
2016.
عدد الصفحات
97 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
الناشر
تاريخ الإجازة
30/5/2016
مكان الإجازة
جامعة أسيوط - كلية الطب البيطري - food Hygiene
الفهرس
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Abstract

Two hundred and forty random samples were collected from different localities in Assiut city including raw milk (90) and some dairy products; including Damietta cheese, Kareish cheese, small scale ice cream , cream and cooking butter (30 samples for each). These samples were examined for the incidence of Stenotrophomonas spp. using Steno medium agar as a selective medium.
The results revealed that 29 (96.67%), 26 (86.67%), 19 (63.33%) out of the examined dairy farms, dairy shops and street vendors milk samples, respectively, were contaminated with Stenotrophomonas spp. Although, both S. rhizophila and S. nitritireducens were the most frequent strains in dairy farms samples in a percentage of 34.48% but S. acidaminiphila was 34.62% in the examined dairy shops milk samples. Moreover, S. nitritireducens could be detected in 47.37% in the isolated strains in street vendor’s milk samples.
As noticed from the findings that 83.33 and 90 % of the examined Damietta and kareish cheese samples were contaminated with Stenotrophomonas spp., respectively. In addition, 52.0% of the recovered strains from Damietta cheese were identified biochemically as S. nitritireducens, while, in kareish cheese was isolated in a frequency of 59.26%. On contrary, lower frequency was recorded for S. rhizophila (20%) in Damietta cheese and S. acidaminiphila (7.41%) in kareish cheese.
In case of ice-cream, cream and cooking butter samples, Stenotrophomonas spp. could be detected in 83.33, 56.67 and 70.00 % of the examined samples, respectively. The highest frequency distribution was recorded for S. nitritireducens from the examined samples.
S. maltophilia was isolated and identified biochemically then by PCR assay. The highest incidence for S. maltophilia was detected for Damietta cheese in a percentage of 13.33% and the lowest incidence 3.33% for each of the examined dairy farms milk, dairy shops milk and cooking butter samples.
The public health significance of Stenotrophomonas in animal and human and preventive measures to improve the keeping quality as well as sanitary conditions of milk and milk products were discussed.