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Abstract Either Thymus vulgaris or Zingiber officinale extract has stimulatory effect on broilers normal growth parameters and immunity. Moreover, affect badly on E.coli O78 strain that attack broilers health. In our search, we aimed to study oral doses of herbal plants (thymus vulgaris or zingiber officinale) on normal and experimentally infected (by E.Coli O78 strain) broiler chickens through evaluation growth indices, leukogram, and biochemical profile as well as some advanced immunological parameters moreover, some histopathological studies on some organs. Firstly, we do preliminary study to confirm the pathogenicity of E.Coli serogroup O78 from 10 day until pathoginicity occurs and choose the ideal experimentally infective dose for the main experiment. Forty (40) Cobb unsexed apparently healthy one-day-old chicks and divided them to 4 equal groups: The first 3 groups were experimentally infected at 10 days old by E.Coli serogroup O78 per Os with 1, 0.5 and 0.25 ml daily of broth containing 3× 10 8 CFU (colony forming units of E. Coli)/ chick. The forth was control negative inoculated with distilled water. We confirm the pathogenisty by the clinical sing appeared after 48 hours that were depression, dropping dullness sleepy appearance, closed eyes, diarrhea pasty vent and inability to stand. The post mortum findings showed congestion in liver, kidney, spleen, intestine bursa of Fabricius and lungs. Pericarditis and perihepatitis were observed. We decided to use the dose 0.5 ml daily of broth containing 3× 10 8 CFU (colony forming units of E. Coli)/ chick in the main experiment as experimenal infective dose. The main experiment was done on 100 chicks all one day old and we divided them into 10 groups. The first group was control negative and the second was control positive. The other eight groups treated by either thymus vulgaris (1 or 2 ml/ 1 litter drinking water) or zingiber officinale (5 or 10 ml/ 1 litter drinking water) from the first day old till the end of the experiment. SUMMARY - 69 -Experimental infection done orally to 4 groups only of the 8 groups at 10 day old |