الفهرس 
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Abstract
The main goal of the present study was the analysis of ~-lactamase production in S.aureus isolates from
- humans and bovine mastitis and the association of lactamase production to methicillin and other antibiotic
resistance.B-lactamase production was assayed using Cefinase disk method and MRSA was detected using
Cefoxitin sensitivity test. The association of ~-lactamase production and basic test results as carotenoid pigment and
hemolysin production was analyzed. The obtained data revealed that, the percentages of J3-1actamase production by
S.aureus strains isolated from bovine milk (cows and buffaloes) were 63.63% and 80%, respectively. Meanwhile, 13-
lactamase production by S. aureus strains isolated from human cases (urine, nasal swabs and blood) was 71.43%,
51.6% and 66.7%, respectively. The results obtained proved that, 59.09% and 60.00% of S.aureus strains isolated
from bovine milk (Cows & buffaloes) were Methicillin-resistant, respectively. While 100%, 70.97% and 50.0% of
Scaureus isolated from human cases proved to be Methicillin-resistant. respectively. Of 86 Surureus isolates
identified 55 (63.95%) produced ~-lactamase of these 39(70.91 %) proved to MRSA and 16(29.1 %) Multi drug
resistant (MDR).The association of r3-1actamase production and MRSA and MDR was discussed. The main goal of
the present study was the analysis of ~-Iactamase production in S.aureus isolates from humans and bovine mastitis
and the association of ~-lactamase production to methicillin and other antibiotic resistance. Overall, there is clear
evidence in this work that p-lactamase producing Svaureus and MRSA can be transmitted between humans and
animals.