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العنوان
Eco-physiological studies on Egyptian and Japanese species of Suaeda martima/
المؤلف
El-Amery, El-Sayed Mohamed Sayed Mohamed Ahmed.
هيئة الاعداد
مشرف / احمد علي الخطيب
ahmed.elkhateb@science.sohag.edu.eg
مشرف / عمر الشيخ
omr.ibrahim@science.sohag.edu.eg
مشرف / احمد علي الخطيب
ahmed.elkhateb@science.sohag.edu.eg
مشرف / عمر الشيخ
omr.ibrahim@science.sohag.edu.eg
الموضوع
species of Suaeda martima, Eco-physiological studies .
تاريخ النشر
2016.
عدد الصفحات
p. 90 :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
28/3/2016
مكان الإجازة
جامعة سوهاج - كلية التربية - نبات
الفهرس
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Abstract

The expression characteristics of superoxide dismutase (SOD; EC 1.15.1.1) isozyme genes from halophytic Suaeda maritima plants native to Japan and Egypt were analyzed using young plants grown under aseptic conditions. A phylogenetic tree based on internal transcribed spacer sequences suggested that Egyptian S. maritima is related to European and India S. maritima, while Japanese S. maritima belongs to a separate clade. An in-gel SOD activity staining assay revealed that leaves from both the Egyptian and Japanese varieties showed high levels of CuZn-SOD and Fe-SOD activity, but no Mn-SOD activity; conversely, stems from both varieties showed Mn-SOD activity as well as other SOD isozyme activities. In Japanese S. maritima leaves, SOD activity was increased by incubation in growth medium containing 400 mM NaCl, while Egyptian S. maritima leaves showed elevated SOD activity in the absence of high salt. Genes encoding Mn-SOD and Fe-SOD were isolated from both plant types. RT-PCR analysis revealed that all SOD isozyme-encoding genes were expressed at the same levels in leaves from both plant types grown in normal or high-salt medium. In contrast, the expression of genes encoding choline monooxygenase and betaine aldehyde dehydrogenase, which