الفهرس 
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Abstract
Major research avenue of the last decade has been to determine
how the immune system benefits from the intake of dietary antioxidants.
Dietary antioxidants are known to increase the immune response. The
best sources of antioxidants for humans and animals are fruits and
vegetables, where flavonoids reach extremely high concentrations.
Flavonoids are not only among the most common antioxidants in nature,
but also those with the highest antioxidant potency in vitro. Phenolic
compounds are a large group of phytochemicals widespread in the plant
kingdom. Phenolic compounds have received considerable attention for
being potentially protective factors against cancer .The mechanisms
underlying this phenomenon are not completely understood, but it is
generally hypothesized that the ingestion of antioxidants may reduce the
deleterious effects of free radicals on the immune response.
The aime of the present study was to evaluate some potential
therapeutic effects of some mixtures fruit juice which rich in natural
antioxidant as flavonoids and phenolic compounds and their effect on
blood antioxidant as an indicator of the situation of immunity.
Using normal and diabetic children and compare the results of it
with control groups and groups was taken chemical antioxidant. Fruits
(plum and grapes) Roselle as herbalists in addition to Honey and sugar
obtained from Samanoud market, Gharbia, Egypt. Juices were extracted
by electric juicing .Chemical composition of juices was determined
(ascorbic acid, Total soluble solids, Ph value, total Phenolic compounds
and total Flavonoids). Chemicales of glutathione-s-transferase,
glutathione reductase and glutathione reduced were obtained from Beta, Alelmy Compan, Mansora, Egypt. The tryptone nutrient agar obtained
from Agriculture faculty- Minufiya University as a gift. One hundred and
twenty children mean weight (50±10 kg) divided into two groups normal
and diabetic the normal group admitted to El Sida Zinab School -Met
Badr, Gharbia, Egypt. where is the diabetic one admitted to Mansoura
university children hospital each groups divided into subgroups male and
female. Every child as taken 100ml of juice daily for four weeks as
follows:-
Group 1: Zero time parameter (8 members).
Group 2: (control Group) drinking no juice (8 members).
Group 3: Antioxidant group) take a dietary supplement Selenium-ACE
tablets effect of selenium, Vit. A, Vit. C and Vit. E,
manufactured by Sigma. (8 children).
Group 4: Drinking juice consists of mix 1.
Group 5: Drinking juice consists of mix 2.
Group 6: Drinking juice consists of mix 3.
Group 7: Drinking juice consists of mix 4.
At the end of the experiment blood samples were obtained in clean
dry centrifuge tubes from vein. Blood samples for hemoglobin were taken
by heparinized micropipette and heparinized micro hematocrit tube for
determination of packed cell volume (PCV). Blood was centrifuged for
10 minutes at 3000 rpm to separate the serum, which were kept in tubes
at –18ºC till analysis. And biochemical analysis was carried out in
Mansoura university children hospital Laboratory, Mansora, Egypt.
Blood samples were used for determination of the
concentration following parameters:
1- Activity of blood antioxidant (glutathione reduced , Glutathione
transferas and redactas).
2- Liver function tests: Glutamate oxaloacetate transaminase (GOT)
and Glutamate pyruvate transaminase (GPT).
3- Kidney function tests: (urea, creatinine).
4- Cbc tests (Hb, Rbcs, Platlets, wbc and Lemph)
5- Blood glucose test.
The obtained results showed that:
CMix3(plum25%