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Abstract In vitro regeneration of common bean (Phaseolus vulgaris L.) is a requirement for genetic transformation, which involves induction and development to the whole plant. Several studies have shown that brassinosteroids attenuate the effects of salt stress in plants. In order to assess the influence of exogenous application of varying concentrations of NaCl and 24-epibrassinolide (24-Epi) each alone or in combination into MS culture media in inducing salt tolerance in vitro P. vulgaris L. plantlets different physiological, biochemical, hormonal and molecular parameters were investigated. The decline in several components of plant metabolism, induced by salt, was attenuated by 24-epibrassinolide application indicating the importance of brassinosteroid synthesis for plants growing under salinity and that 24-Epi improvement might have contributed to the salt tolerance. |