الفهرس 
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Abstract
Abstract
WSSV is one of the serious viral diseases that affecting shrimp industry and causes serious economic losses due to 100% mortality within 2-10 days. This study was performed to diagnosis of WSSV in Egyptian shrimp by histopathological examination and molecular based methods (PCR). A total of 90 shrimp samples were collected from the local Egyptian fish market located at three coastal provinces. Twenty infected and 10 apparently healthy shrimp from each province were investigated. Three shrimp species of Farawla, white and Gazaz shrimp (Aristeus antennatus, P.semisulacatus, Penaeid japonicas) were selected from three corresponding coastal provinces (Ezbit El-Borg in Dumiatta, Al Areesh in Nourth Sinai and Al Burlis city located at Al Burlis lake, Kafr El-Sheikh) respectively. All shrimp samples were exposed for postmortem examination and collection of internal organs and tegument. A part of cuticle, gills, heart, hepatopancreas, lymphoid tissues, stomach and gut were collected for PCR and molecular diagnosis of white spot syndrome virus and the other part were preserved in Davidson’s Solution for histopathological examination that showing hypertrophied nuclei with marginated chromatin, basophilic intranuclear inclusion bodies, hemocytic infilteration, cellular degeneration, necrotic area characterized by vacuolization, pyknosis and karyorrhexis, hyaline degeneration of haematopoietic tissues and Muscular layer showed multifocal necrosis. Molecular Analysis (PCR) by using specific Primer for WSSV (VP28 gene), visualization of predicted band at 677 bp in the three studied shrimp species (Penaeus Japonicus, Penaeus semisulcatus and Aristeus antennatus). from the obtained results we can conclude that WSSV might infect the Egyptian shrimp and circulate among different shrimp species in the coastal provinces. PCR assay proved to be specific and sensitive test for direct diagnosis of WSSV in infected shrimp samples compared to the non-infected apparently healthy shrimp as being confirmed by gross and histopathological examination.