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العنوان
Comparative Study between Sandwich ELISA, Dot-ELISA and Immunomagnetic-Beads-ELISA
Techniques in Diagnosis of Schistosomiasis
haematobium /
المؤلف
Yousif, Ahmed Badawi.
هيئة الاعداد
باحث / أحمد بدوى يوسف
مشرف / عبد الحميد عبد التواب
مناقش / ابراهيم ربيع بيومى
مناقش / مروة أحمد الدرديرى
الموضوع
Comparative study of electoral systems.
تاريخ النشر
2015.
عدد الصفحات
134 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علم الأحياء الدقيقة (الطبية)
تاريخ الإجازة
21/1/2015
مكان الإجازة
جامعة الفيوم - كلية الطب - قسم علم الطفيليات
الفهرس
Only 14 pages are availabe for public view

from 134

from 134

Abstract

mmunodiagnosis of urinary schistosomiasis has proved to be
very useful and sensitive. Antigen detection assays are
considered of prime importance for immunodiagnosis, as the
detection of circulating antigens can indicate an active infection.
The present study described the immuno-affinity purification of
cysteine proteases (CP) from ES products of living S.
haematobium eggs after being cultured in RPMI 1640 medium.
Purification was done by gel filtration chromatography on
sephadex A-50 (DEAE column chromatography method)
followed by gel filtration chromatography on sephacryl-S-200
HR column. The eluted proteins, from the gel filtration column
chromatography analyzed by 12.5% SDS PAGE under reducing
condition, showed only one band at 27.5 kDa which was CP.