الفهرس 
List of abbreviationsOnly 14 pages are availabe for public view
 |  | from | 185 |  |  |
| | | from | 185 | | |
Abstract
C.violaceum (C. violaceum ) is a Gram-negative facultatively anaerobic proteobacterium, slender, slightly curved, rod-shaped bacteria, C.violaceum infecting immune compromised individuals who are hospitalized and suffer from severe underlying diseases, the main features in most of the cases with fatal outcome seem to be sepsis, multiple liver abscesses and diffuse pustular dermatitis. The aim of this study to detect the frequency of C. violaceum causing nosocomial infections in Mansoura University Hospitals with detection of different factors increase the risk of their acquisition. Also investigation of the morphological, cultural and biochemical characteristics of isolates to be recovered from the various samples by comparing the appropriate descriptions isolates in order to confirm identification, also Micro-scan identification system ( DADE BEHRING ) is used for confirm identification, also tracing to source of infection via Screening of health care worker, screening of instruments in common use and environment surrounding, also detect the most effective drugs for treatment by antibiotic susceptibility test . Also to develop a PCR-based assay and other molecular technique such as RFLP and RAPD-PCR to specifically detect, differentiate and grouping among different isolates of C. violaceum. Clinical samples were collected, regardless the type of infection and the sex of patients, This study was carried out over a period of 26 months from August 2010 to October 2012. During that period 2657 samples were collected from different hospitals in Mansoura University. Out of 2657 isolated samples, three species of C. violaceum were recovered from males, one from blood and two from wound in Plastic surgery and burn from different Mansoura University Hospitals. By tracing to source of infection via Screening of health care worker, screening of instruments in common use and environment surrounding, one bacterial isolate of C. violaceum discovered from hospital floor, this may be the source of infection. Burn injury is major risk factors for getting hold of nosocomial C. violaceum infections. This may be attributed to the injury of epithelial cells that facilitate the invasion process. Phenotypic characterization was performed using cultural characteristics and morphological appearance in Gram stained smears and conventional biochemical reactions. Microscane automated system was used to confirm the bacterial isolates of C. violaceum.