الفهرس 
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Abstract
It is a great important to discover new antimicrobial agents as the human health and economy suffer from the development of infections caused by microbial pathogens, including multidrug resistant bacteria. The aim of the current research is to assess the photo-thermal treatment (PTT) of gold nanoparticles (AuNPs) versus the photodynamic Treatment (PDT) of Toluidine Blue (TBO) on Escherichia coli (E. coli) and Bacillus cereus (B. cereus) cells. Photodynamic effect of TBO using different doses of He-Ne laser and photothermal effect of AuNPs using green light LED 530 nm) were studied for bacterial eradication. AuNPs were prepared with reduction method without capping (AuNPs-cit) and its biological effect was studied versus, AuNPs capped either with polyvinyl alcohol (AuNPs -PVA) or with Polyvinylpyrrolidone (AuNPs -PVP). Results showed that, 90 .M TBO has strong lethal effect on bacterial cells after irradiation with 2.4 J/cm2 He-Ne laser. AuNPs PVP alone has no effect on bacterial cells while AuNPs-cit and AuNPs-PVA inhibit the bacterial growth. Moreover, 0.9 J/cm2 from LED inhibit the growth of E. coli in the presence of AuNPs-PVP. Finally it was concluded that, both PDT and PTT considered as effective methods for bacterial eradication. Capping material of AuNPs plays an important role in the biological effect of AuNPs whereas some of them are not biocompatible.