الفهرس 
English coverOnly 14 pages are availabe for public view
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Abstract
Our present work aimed to explore the application of gold nanoparticles in tumor diagnosis and therapy. Cancer is a public leading cause of death in the world. Searching for efficient method for diagnosis of cancer and selective therapy are extremely active field today. With the great development of nanotechnology, various nanoparticles in different structures, shapes and composites provides good potential for their application in cancer diagnostics and therapy. Compared to other nanoparticles (NPs), gold nanoparticles have great advantages in cancer applications due to their easy preparation, efficient bioconjugation, potential noncytotoxicity,tunable and enhanced absorption properties. The strong absorption of gold nanoparticles provides an opportunity for their use in photothermal therapy. By irradiation with visible laser, the gold nanoparticles absorb light strongly due to Plasmon resonance, and then this light is transferred efficiently into heat due to irradiative processes (heat dispassion to the surrounding).Moreover the use of nanoparticles as drug delivery vehicles for anticancer therapy has great potential on the future of cancer therapy. As tumor architecture causes nanoparticles to preferentially accumulate at the tumor site, their use as drug delivery vectors results in the localization of a greater amount of the drug load at the tumor site; especially due to its huge surface area; thus improving cancer therapy and reducing the harmful nonspecific side effects of chemotherapeutics.In this work gold nanospheres proved to be highly effective photothermal agent and drug carrier.Gold nanospheres have been prepared by chemical method and the photostability of the formed particles has been studied.AuNPs incubated with MCF7 breast carcinoma cell line for 24 hours and exposed to DPSS (Diod Pumped Solid State) laser 532 nm with a power of 250mW decrease cell viability to 66%.Moreover the binding of the anticancer drug doxorubicin hydrochloride with gold nanoparticles (AuNPs) has been rified using various analytical techniques (UVVisible absorption spectroscopy, fluorescence spectroscopy and FTIR spectroscopy).Subsequently the in vitro study of doxorubicin coated on AuNPs showed high efficiency on MCF7 viability reduction more than free doxorubicin.The in vivo study of doxorubicin coated on AuNPs on mice bearing tumor reveal increase in the survival period and decrease in the tumor growth comparing to treatment with free doxorubicin.For tissue diagnostic purpose FTIR (Fourier Transform infrared spectroscopy) have been used.The experimental conditions such as nanoparticle preparation, laser power, time of exposure, and concentration of the drug which caused the best efficiency of breast cancer cells viability reduction have been studied.Key words: Gold nanoparticles, cancer diagnosis and treatment, breast carcinoma cell line,doxorubicin hydrochloride, Diod Pumped Solid State laser, Photothermal therapy, drug delivery.