الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 32 |  |  |
| | | from | 32 | | |
Abstract
Due to the importance and versatility of benzimidazole ring as an important pharmacophore and a privileged structure in agro chemical industry make it an important point in many researches. Moreover, considerable interest has been focused on dithioates which are known to be active as antimicrobial agents.
Therefore, a combination and merging of both benzimidazole and a moiety of dithioate as well as dithioate containing two sulfur atoms had attracted the attention in order to design new bioactive molecules higher than both moieties individually. Thus, a novel synthesis of new carbendazim dithioate analogs 156163 was presented. Reaction of 2-aminobenzimdazole 8, carbonyl sulfide, sodium carbonate and the appropriate alkyl halide were dissolved in DMF and stirred for 48 h at room temperature to afford benzimidazole dithioate (carbendazim sulfur analogs) 156-163 in good to excellent yields. The chemical structure of all the synthesized compounds were elucidated on the basis of their spectral data IR, 1H, 13C NMR and Fourier transform Ion Cyclotron Resonance (MALDI), PE SCIEX API Q-Star Pulsar (ESI).
All the synthesized carbendazim dithioate analogs were screened for in vitro and in vivo antimicrobial activity (antibacterial, antifungal and soil dehydrogenase activity). Some of them possess high potent activity against the Gram-negative and Gram-positive bacteria. The antifungal activity has been found less than the antibacterial activity of the all synthesized carbendazim sulfur analogs. The most sulfur analogs showed slight activity against Fusarium solani and Fusarium oxysporum. But most analogs showed inhibition hyphal growth of Fusarium oxysporum than Fusarium solani. With increasing soil pollutants resulting from excessive use of pesticides like carbendazim, it is necessary to detect the effect of novel carbendazim analogs in soil dehydrogenase which act as indicator for all life process in soil. Investigation of the dehydrogenase activity of soil for sulfur analog 159 showed the highest suppressive activity against the soil microorganisms compared to all carbendazim and the other sulfur analogs. Whereas, some of the sulfur analogs possess the same effect on the reduction of dehydrogenase activities as carbendazim.