الفهرس 
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Abstract
The aim of the present study was to investigate serum uric acid level and total antioxidant capacity and its potential usefulness as a markers of oxidative stress in children with β-thalassemia major as well as to asses the relationship between them and various clinical and laboratory characteristic of recruited patients.
This was a prospective observational study conducted at the hematology clinic, Beni-suef hospital, faculty of medicine, Beni-suef university. thirty five patients with β-thalassemia major and fifteen apparently healthy age and sex-matched subjects were included in the study and served as a control group. Patients consisted of 18 females(51.4%) and 17 male (48.6%) their age ranged from 2 years and 14 years (mean age was 8.2 ±4 years) and were diagnosed as betathalassemia major based on conventional clinical and hematological criteria. Patients older than 14 years old, those who have other disease than beta-thalassemia were excluded from the study.
All individuals in this study were subjected to full history talking and complete physical examination. Complete blood count, serum ferritin, serum uric acid and serum total antioxidant capacity were measured by different methods.
Serum uric acid level in healthy non anemic children ranging from 2.7mg/dl to 5.8 mg/dl with mean value 4.2 mg/dl and this level was lower compared to cases with betathalassemia major with mean serum uric acid level was 10.5 mg/dl (range from 5.88 to 17.3 mgldl).
Serum total antioxidant capacity in btathalassemic children ranged from 1.1 mM/L to 2 mM/L with mean 1.6 mM/L and this level was lower compared to healthy non anemic children where mean serum total antioxidant capacity 2.1 mM/L (range 2 to 2.3 mM/L)
Patient group had significant higher serum uric acid compared to control group (p=0.001) but they had significant lower serum total antioxidant capacity level compared to control group (p=0.001).
Among the patient group mean serum ferritin level was 863.6 ng/ml ranged from 460 ng/ml to 1390 ng/ml and this level was higher compared to control group where mean serum ferritin was 127.4 ng/ml (range 76 to 186 ng/ml).
Serum total antioxidant capacity had negative correlation with frequency of blood transfusion (r=-0.2) but not statistical significant (p=0.562) and with serum ferritin (r=-0.2).
Patient group had higher serum ferritin compared to control group (p=0.001). According to complete blood count patient group had significant lower Hb level(p=0.001), MCV (p=0.002) ,MCH (p=0.001),MCHC (0.012) and platelets (p=0.002) compared to control group. Also group A(non splenectomized) patients had lower platelets(340.1 ± 158.0) and WBCs (8.5±2.2) compared to group B (splenectomized) patients(571.2±198.0)
( 17.8±9.8) respectively.
Serum total antioxidant capacity showed significant dependence on serum ferritin (Sig=0.012) and age of patient (Sig=0.013) compared to control group in linear regression analysis. And also it had lower concentration in patient receiving Deferiprone chelating therapy compared to patient without Deferiprone .