الفهرس 
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Abstract
In the in vitro study, two breast cancer (BC) cell lines were used, human non-metastatic BC cell line (MCF-7) and murine metastatic BC cell line (4T1) to study the chemo-sensitizing effect of MGN-3 on Taxol treatment. We measured the cell viability and proliferation using both MTT assay and trypan blue assay to determine the half maximal inhibitory concentration (IC50) of the two cell lines after both 24 and 48 hours of incubation time with a serial dilution of MGN-3 and Taxol. Data of the in vitro study indicated the potential of MGN-3 to enhance the anti-cancer effects of paclitaxel via reducing the concentration required for killing cancer cells. The IC50 value for paclitaxel was reduced by over 100-fold for both MCF-7 and 4T1 cells in the presence of MGN-3. In the in vivo study, 80 female Swiss albino mice (2-months old, each weighing 19- 20g) we used and Ehrlich Ascites carcinoma cell line as a breast cancer model to study the tumor-inhibitory effect of MGN-3/Biobran plus Taxol in mice bearing breast carcinoma tumors and to elucidate the mechanistic basis of the chemo-sensitizing ability of MGN-3/Biobran. We applied two different dosage of Taxol: low dose (Taxol-L, 2mg/kg); and high dose (Taxol–H, 10mg/kg), while MGN-3 dosage was 40 mg/kg BW. On day 0 of tumor cell inoculation, mice were divided into 8 groups. In conclusion, this study demonstrated that treatment with MGN-3/Biobran increased the susceptibility of different breast cancer cells to Taxol in vitro and in vivo. When solid tumor-bearing mice were treated with Taxol in combination with MGN-3/Biobran, tumor growth was significantly inhibited, whereas the single-agent activity of Taxol alone was less effective. This suggests that MGN-3/Biobran, extracted from rice bran, may be used as a chemo-sensitizer to enhance the therapeutic effect of Taxol in the treatment of metastatic and non-metastatic breast cancer.