الفهرس 
IntroductiionOnly 14 pages are availabe for public view
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Abstract
Bacteriological examination of 246 samples of food of animal origin resulted in 223(90.65%) positive samples for Gram-negative bacteria by isolation of 319 Gram-negative bacteria, 274 for Enterobacteria (85.89%) and 45 for Pseudomonas (14.10%). The most isolated Enterobacteria were Klebsiella species (34.95%); followed by Escherichia coli (26.01%); Citrobacter spp. (16.26%); Enterobacter (15.04%); Proteus spp. (10.56%); Serratia spp. (6.91%) and Salmonella spp. (1.62%). Pseudomonas was biochemically identified as Ps. alkaligenes (8.94%); Ps. aeruginosa (5.28%) and Ps. stuzeri (4.06%). The study included serological identification of the isolated Salmonella strains and ten representative E. coli strains. Antibiogram study of the isolated E. coli showed 100% sensitivity to ciprofloxacin 5ug. and 100% resistance against 4 tested antibiotics. Antibiogram study of the isolated Salmonella strains showed 100% sensitivity polymyxin B 300 units and 100% resistance against the most tested antibiotics. The molecular study included detection of the eaeA (protein intimin), iutA (encoding aerobactin) and iss (increased serum survival protein) gene factors that may be expressed in the examined E.coli serovars and detection of the specific DNA bands for invA (Salmonella invasion protein), stn (enterotoxin), avrA (recombinant protein) and SopB (effector protein) gene factors of the examined Salmonella serovars.