الفهرس 
Introduction and aim of workOnly 14 pages are availabe for public view
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Abstract
Hippocampus is a part of the limbic system of the brain, which is located between the diencephalon and the cerebral neopallium. The hippocampus is an example of the simple three layered allocortex. The hippocampus proper and the dentate gyrus each consists of only one layer of principle neurons – the pyramidal cells and granule cells respectively.
Twenty four pairs of male and female albino rats were used in the present study. The rats were put in separate cages, each contained one male and one female rat. They were kept for mating for 24 hours. The next day, female rats were examined for vaginal plug of semen. The first day of conception was determined if the vaginal plug is positive and considered to be E1.
Pregnant female rats were kept separate in cages and were followed up for pregnancy dating. from E16-E21, two or more pregnant rats were taken for each day of age, and then sacrificed by decapitation. The abdomen and the uterus were opened up, and then the embryos were removed, heads were separated and injected by Bouin fixative through making pores in four directions at the margin of skull cap.
from postnatal ages P1, P7, P14, P21, P30 and P90 (adult), three rats were taken for each age and sacrificed by decapitation. The skulls were opened, the brains were removed and the hippocampus was removed.
Either the head (E16-E21), or the hippocampus (P1-adult), was kept in Bouin’s fixative for 24 hours, then transferred to 10% neutral formalin and stained by Haematoxylin and Eosin.