الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
 |  | from | 116 |  |  |
| | | from | 116 | | |
Abstract
Gentamicin has potent nephrotoxic side effect. Nephrotoxicity by gentamicin leads to production of reactive oxygen species ROS which leads to cell death in renal tubules and thus disturbing all physiological functions of the kidneys including renal filtration, reabsorption of small and large blood molecules essential for the body, osmotic and pH balance in blood and hormone productionAn experiment was carried out to justify the curative effect of alpha lipoic acid on the acute renal injury artificially induced in male rabbits and also testing comparatively the effect of withdrawal recovery for 14 day periodTwenty five (25) male rabbits aging 3 months, weighing 2250-2690 g, were divided a 5 groups, and each individual rabbit was housed in a separate cageGroup 1 was non treated control, group 2 received gentamicin for 7 days at 80 mg / kg B.W. for 7 consecutive days, i.m., group 3 was treated as group 3 then treated by alpha lipoic acid for 14 consecutive days at oral dose of 50 mg / kg B.W., group 4 was treated as group 2 followed by 14 days without any treatment to test the effect of withdrawal recovery on animals. <This study aimed also at studying the biochemical, physiological, histopathological and genetic indicators for the acute nephrotoxicity induced by gentamicinOur results showed that supra-therapeutic dose of gentamicin at 80 mg / kg B.W. for 7 consecutive days caused severe biochemical, physiological, hematological, histopathological and genetic alterations. Gentamicin toxic dose treatment in group 2 caused a decrease in the specific gravity from 1.020 to 1.010 compared to the non toxicated groupWhile lipoic acid after gentamicin treatment and withdrawal recovery for 14 days elevated the specific gravity to 1.015No deaths were recorded in the groups of rabbits that were given gentamicin for 7 days at 80 mg/kg B.W. The body weight was decreased by 200 g for each animal compared to the negative control. Also rabbits of group 4 left for recovery for 14 days after gentamicin were still having B.W. less than the weight of each animal in group 3 those treated by alpha lipoic acid for 14 days by 487 g. Under the effect of supra-therapeutic dose of gentamicin administered to 3 months old male rabbits at a dose of 80 mg / kg B.W. i.m. for 7 days for induction of acute renal injury, the following biochemical parameters were elevated significantly compared to the negative non treated controlsSerum P, K, tissue MDA, total protein (TP) in urine, serum cholesterol, triacylglycerol, high and low density lipoproteins, serum ALT and AST and LDH and the GGT in urine and albumin in urine as well as albumin / cratinine ratio which was drastically increasedAll the above mentioned biochemical parameters were decreased significantly after the use of lipoic acid for 14 daysAlthough there was an improvement in the values of these parameters after withdrawal recovery for 14 days, but the improvement achieved by alpha lipoic acid were much stronger than withdrawal recovery for 14 daysadministered to 3 months old male rabbiOn the other hand, under the effect of supra-therapeutic dose of gentamicin ts at a dose of 80 mg / kg B.W. i.m. for 7 days for induction of acute renal injury, the following biochemical parameters were lowered significantly compared to the negative non treated controlsSerum total protein, globulins and albumin, tissue CAT activities and tissue GSH, superoxide dismutase SOD contents, blood glucose, serum Ca and serum Na. In urine; creatinine and the specific gravity of urine were also lowered. Also by toxic dose of gentamicin there was a severe and marked fragmentation of the DNA where the DNA was represented by several bands of no specific molecular weight and of no fixed dimensions. Also the degenerative picture of renal tubules was documented, and the alpha lipoic acid treatment corrected these acute changes. Simillarily leaving for withdrawal recovery was useful in alleviation of these histologic alterations but was not as good as alpha lipoic acid We can conclude that treatment by alpha lipoic acid for 14 consecutive days, through daily oral dose of 50 mg / kg B.W. seemed from the biochemical, histopathological and genetic evidences to be effective treatment for the induced acute kidney injury by gentamicin (formerly; induced acute renal failure)Also, there was an improvement in the values of these parameters after leaving without any treatment for withdrawal recovery for 14 days, but the improvement achieved by alpha lipoic acid were about 4-5 times stronger than withdrawal recovery for 14 days.