الفهرس 
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Abstract
Three field and laboratory immunological diagnostic kits were developed to multiple detection of microbial causes bovine mastitis. Two lab diagnostic kits were Double Sandwich and Dot ELISA. While the third was field diagnostic kit thus lateral flow devices (LFD) were developed. The minimal microbial counts to be give positive of Double Sandwich ELISA, Dot ELISA and Lateral flow device were (100CFU/100µl), (1250CFU/ 100µl) and (100CFU/100 µl) respectively. The sensitivity, specificity and accuracy of double sandwich ELISA as compared to bacteriologically examination were calculated and was found to be 93.7%, 97.6% and 96%, respectively, for E. coli; 82.6%, 94.2 % and 90.6% respectively, for S. aureus. It was 93.7 %, 98.3 % and 97.3%, respectively, for K. pneumoniae On the other hand the sensitivity, specificity and accuracy of Dot ELISA as compared to bacteriologically examination were calculated and was found to be 93.7%, 97.6% and 96%, respectively for E. coli, 75%, 100% and 89.3%, respectively for S. aureus it was 69.5%, 96.1% and 88%, respectively for K. pneumoniae. The sensitivity, specificity and accuracy of LFD as compared to bacteriologically examination were calculated and was found to be 93.7%, 95.3% and 94.6% respectively, for E. coli; 82.6%, 92.3% and 89.3% respectively for S. aureus it was 94.1%, 98.2% and 97.3% for K. pneumoniae. The pretesting treatment of the milk samples with of application buffer associated with its pre-incubation in TSB for 6hr at 37°C increased significantly the sensitivity results of three prepared diagnostic kits.