الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 183 |  |  |
| | | from | 183 | | |
Abstract
Environmental pollution is a worldwide problem and its potential to
influence the health of human populations is great. Radiation and 1-
nitropyrene are different sources of pollution that affects human
health through production of reactive species, free radicals and
formation of DNA adducts. Our study examined the ability of natural
antioxidant caffeic acid phenethyl ester in protecting against
oxidative stress produced by radiation and/or 1-nitropyrene.
The results of the present study can be summarized as follows:
1- In experiment 1: rats exposed to 6Gy fractionated dose
showed a profound decrease in ALP and ALT levels, and a
significant increase in AST, γ-GT and LDH. While, according
to the oxidative stress profile, liver MDA level increased and
depletion of lung GSH was observed. Concerning trace
elements; Zn, Cu, and Mn concentrations decreased in liver,
while the concentration of Fe increased. Histopathological
examinations showed severe inter- and intra- cellular edema
and deformation of mitochondria in liver cells, while in lung
cells, we observed haemorrhage and excessive amounts of
collagen, indicating for fibrosis.
2- In experiment 2: rats administered 600mg/kg cumulative
dose of 1 -NP attenuated an increase in ALP, ALT, AST, γ-GT
and LDH. MDA level increased in both lung and liver, while
GSH was depleted in both organs. Noticeable decrease in the
concentrations of liver Zn, Cu and Mn were observed.
Examination of liver and lung tissue histopathologically
revealed deformation of mitochondria, capillary dilatation, as
well as presence of polymorphes in liver tissue. While, in lung
5. Summery and conclusion
tissue deformation of mitochondria and excessive fibroblasts
were observed.
3- In experiment 3: rats exposed to 6Gy fractionated dose in
addition to administration of 600mg/kg cumulative dose of 1-
NP have shown similar results to that exposed to radiation
only regarding biological parameters, except for the
concentration of Cu and Mn. As for histopathological
examination, cumulative damage of both radiation and 1-NP
was observed in both lung and liver tissue.
4- Treatment with CAPE has shown clear ameliorative effect
to the oxidative stress damage produced by irradiation and/or
1-NP in all measured liver enzymes to different extents.
Concerning the oxidative profile, CAPE decreased the level
of MDA and increased the concentration of GSH in both lung
and liver tissue. In histopathological examination, CAPE only
had a partial ameliorative effect to the histological damage
produced.
It was found in this study that administration of cumulative dose of
60 µmol/kg CAPE was effective in reducing irradiation and/or 1-NP
pollutants damage. It could be concluded that CAPE might scavenge
or neutralize free radicals, increase the levels of enzymatic and nonenzymatic antioxidants, chelate and disarm the oxidative properties
of some metal ions. So, CAPE can exert beneficial protective
potentials against radiation and chemically induced biochemical
perturbations and disturbed oxidative stress markers thus it could be
recommended as a promising approach for raising the antioxidative
profile