الفهرس | Only 14 pages are availabe for public view |
Abstract Cucumber mosaic virus (CMV) was isolated from cucurbits plants, collected from many forms in Ismailia governorate. The infected plants showed mosaic or mottling on the leaves, which were stunting and yellowing. Samples were inspected serologically for CMV using direct ELISA. Particles of virus belonging to genus of Cucumovirus (family Cucumovirida) are isometric, 29 nm in diameter, containing 8621 bp RNA. The aim of this study is to isolate and identify CMV depending upon; host range, virus stability, virus transmission, purification, antiserum production, serological tests, electron microscopic investigation, in addition to the effect of CMV on anatomical structure, and protein content. Sequence of CMV strain and comparing it with other CMV strains were studied. The partial nucleotide sequence alignment showed (95%) homology between the CMV New Zealand isolate and CMV-Egyptian isolate. The sequence alignment also indicated that the CMV isolate is far from similarity with India CMV, showing (92%) according to the phylogenetic homology tree. Key words : Cucumber Mosaic virus – Biological characteristic - Molecular characteristic - Full genome sequence.- isolates relatedness. |