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Abstract The present thesis is devoted for developing new methods for the analysis of some drugs affecting gastrointestinal tract, namely: itopride hydrochloride, silymarin, DDB, omeprazole and famotidine, either in pure forms or in pharmaceutical preparations. A sensitive and rapid spectrofluorimetric method was introduced for determination of itopride in raw material, tablets as well as in the presence of co-formulated drugs. The method was extended to investigate the inherent stability of the drug after exposure to different stress degradation conditions. The hepatoprotective drugs; silymarin and DDB were simultaneously determined by two analytical methods including; first derivative spectrophotometric and micellar liquid chromatographic methods. The method was applied for the determination of the studied drugs in their raw materials and combined capsules. A reversed-phase liquid chromatographic method with fluorescence detection was developed for determination of DDB in raw material and pills. The method was further applied to content uniformity testing and in vitro dissolution test according to USP guidelines. A simple and rapid isocratic HPLC method with UV detection was developed for simultaneous determination of omeprazole, tinidazole and doxycycline in their raw materials as well as combined capsules A ternary mixture of famotidine, paracetamol and diclofenac was simultaneously determined by HPLC method with UV detection. The method was applied for the determination of famotidine in different pharmaceutical formulations. Simple, rapid and precise spectrophotometric and precolumn derivatization HPLC methods for estimation of famotidine in raw material and pharmaceutical formulations through derivatization with sodium nitroprusside. The validation criteria of the developed methods were intensively studied. All the results were statistically analyzed and compared with those given by official or comparison methods. |