الفهرس | Only 14 pages are availabe for public view |
Abstract Salmonella is considered the most important bacterial diseases that infect poultry and transmitted to human. Isolation of Salmonella from tissues of poultry needs firstly inoculation of the tissues in enrichment media that inhibit growth of bacteria other than Salmonella and helps in propagation of Salmonella. By testing several enrichment media as RV, TT and SF, it was found that RV is the best one. Serotyping of Salmonella isolates is the most important steps necessary for Salmonella identification, but because of the numerous difficulties in this method, the use of molecular biology techniques in achieving this goal is very imprtant. PCR and sequencing of inv A gene were used for Salmonella identification. Through molecular biology and informatics and comparison with data on Gene- Bank, 36 isolates of Salmonella were identified to 8 S. Entritidis, 18 S. Heidelberg, 6 S. Gallinarum and 1 S. Newport, there were 3 isolates untyped. Serological test for 12 isolates have been selected randomly, the results revealed that, 9 S. Infantis , 2 non-Salmonella bacteria and 1 S. Newport, While by molecular techniques of these isolates were 8 S. Heidelberg, 2 S. Gallinarum and 1 S. Newport. Therefore, this conflict needs more investigation. In addition, in this study, antibiotic sensitivity test was performed, S. Heidelberg isolates considered as multidrug resistant isolates by 94.4 %, which is dangerous to human health when transmitted from birds to human. Antibiotic residues of tetracyclines and β-lactam were detected but no aminoglycosides or quinolones were detected, because slow absorption of aminoglycosides and non-use of quinolones in farms used in this study. These results make us recommend using antibiotics, which with low absorption as growth promoters or prophylaxis. Detection of resistance gene (tet A) of tetracyclines and (bla TEM & bla SHV) of β-lactam by PCR، to find a relation between antibiotic resistant Salmonellea and antibiotic residues in their tissues. |