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Abstract A sample of 60 vital permanent premolars and 36 non-vital second primary molars in children aged 12 to 14 years for group I and 6 to 8 for group II were selected from the patient population at the dental clinic of pediatric dentistry department-faculty of dentistry-Suez canal university . Diagnosis was performed and the children were examined both clinically and radiographically to be allocated to two main groups: group I : That included sixty vital premolars teeth that extracted bilaterally for orthodontic treatment. In this group vital pulpotomy done and subdivided according to the material used in to 3 subgroups (ozonated water 1250, 2500 ppm and calcium hydroxide). group II: That included thirty six second primary molars indicated for non-vital pulpotomy treatment and according to the material used it is subdivided in to 3 subgroups (ozonated water 1250, 2500 ppm and formoeresol). For group (I) the premolar teeth extracted and histolgical assessment was done for group (II) assessment was done clinically and radiographically. Summary and Conclusion Results of the present study showed that for group (I) marked and outstanding feature was the increased vascularity and dilatation of the blood vessels which was so marked with the higher concentration of ozone. However some specimen were reported with degeneration of odontoblasts, dissociation of collagen fibers and reticular atrophy of the pulp tissues. For the calcium hydroxide subgroup the pulp tissue showed minimal degeneration changes manifested as cytoplsmic vaculization, more or less normal appearance of collagen fibers with minimal dissociation, however the calcific deposits appeared larger. For group (II) rate of success both clinically and radiographically was higher for ozonated water of higher concentration (2500 ppm) in comparison to both formocresol and ozonated water of lower, concentration (1250 ppm). RECOMMENDATIONS |