الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
 |  | from | 153 |  |  |
| | | from | 153 | | |
Abstract
The aim of this study is to evaluate the Mycoplasma Duo kit as a rapid method for detection of ureaplasma species in endotracheal aspirate samples from respiratory distressed premature neonates compared to conventional culture media. Also to determine its sensitivity and specificity .
Under complete aseptic precaution paired endotracheal aspirate samples were taken from each of the 60 premature neonates (less than 35 gestational weeks) and suffering from respiratory distress and mechanically ventilated in neonatal intensive care unit in BenhaUniversityHospital. The collected samples were transported in Ureaplasma transport media to Microbiology laboratory without delay. The consent was taken from neonate’s parents before taking the samples.Each pair of the collected samples were examined for the presence of ureaplasmaby:
1) One sample was inoculated into ureaplasma agar and broth media and incubated under microaerophilic conditions at 37°C for 2-5 days. The growth was identified as ureaplasma according to (Marmion and Harris.,1996).
2)The other sample was tested for the presence of ureaplasma using a commercially available Mycoplasma Duo kit(Biorad ultradiagnostics)according to manufacturer’s instructions.
In this study out of 60 respiratory distressed premature neonates, ureaplasma is detected in 20 (33.33%) cases by both ureaplasma agar and broth cultures and in 22 (36.67%) cases by Mycoplasma DUO kit. Among these 22 cases , ureaplasma with a high titre ( U ≥104) is detected by Mycoplasma Duo kit in 19 (31.67%) cases.
Ureaplasma and M.hominisare both detected in 3 (5%) cases by Mycoplsma DUO kit (Ureaplasma with low and high titre and M.hominis with low titre). Only one of these three cases is positive for M.hominis with high titre(H ≥104) . M.hominisisn’t detected alone by Mycoplasma Duo kit.
In this study the sensitivity and specificity of ureaplasma detection by Mycoplasma DUO kit versus both ureaplasma agar and broth cultures are 100% and 95% respectively and the positive and negative predictive values are 90.91% and 100% respectively. Mycoplasma DUO kit is accurate ( Accuracy=0.9750) ascomparedwith Ureaplasma agar and broth cultures.
In 20 ureaplasma positive cases by both ureaplasma agar and broth cultures the incubation time (in days) taken to get positive results for ureaplasma was as follows( 2 cases after 2 days, 5 cases after 3 days, 8 cases after 4 days and 5 cases after 5 days) of incubation.
In 22 ureaplasma positive cases by Mycoplasma Duo kit the incubation time (in days) taken to get positive results for ureaplasma was as follows( 19 cases after 1 days and 3 cases after 2 days) of incubation.
Regarding the incubation time taken to get positive results by both ureaplasma agar and broth cultures is ( 2-5) days with mean ± SD (3.8 ± 0.95) while it is (1-2) days with mean± SD (1.14 ± 0.35) by Mycoplasma Duo kit. There is a highly significant difference (P-value<0.001) as regard incubation time taken to get positive results by both tests.Mycoplasma Duo kit is more rapid than ureaplasma agar and brothcultures for detection of ureaplasma.
This study shows that there is no statistical significant differenceas regards sex differences (P value>0.05) between positive and negative cases of ureaplasmas by both ureaplasma agar and broth cultures and Mycoplasma DUO kit .
Regarding average gestational age there is a statistical significant difference between positive and negative cases of ureaplasma using both ureaplasma agar and broth cultures (P value=0.02) and Mycoplasma DUO kit (P value=0.04) .
For average birth weight this study shows that there is a statistical significant difference (P value=0.04) between positive and negative cases of ureaplasma using Mycoplasma DUO kit and there is a borderline statistical difference (P value=0.06)between positive and negative cases of ureaplasma by both ureaplasma agar and broth cultures.