الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
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Abstract
Summary and Conclusion
B-CLL represent the most common type of adult leukemia in western societies and is characterized by absolute lymphocytosis with painless lymph node enlargement and prolonged chest infection or pneumonia with accumulation of anergic, self reactive mature CD5+ B cells in the blood and expression of CD19, CD20, CD23 and CD27. Also genomic aberrations can now be identified in approximately 80% of B-CLL patients.
CD160 is a glycophosphatidylinositol (GPI)- linked membrane protein, it recuits phosphoinositide-3 kinase (PI3K) and induces phosphorylation of Akt and extracellular signal- related kinase down stream signaling elements in NK cells . In CD8+ T cells, CD160 enhances CD3- induced proliferation and improvees CD3 –inducd cytotoxicty (Rabot et al ., 2007) .
I n healthy people , CD160 distribution is restricted to both circulating and tissue cytotoxic NK cells as well as T cells, with no expression in normal B cells . In disease state ,CD160 is up regulated on CD3+ CD8+ T cells during infection with human immunodeficiency virus and is expressed on neoangiogenic vasculature of tumors as well as CD4+ T cells infilterating inflammatory skin diseases (Abcassis et al ., 2007).
The aim of the study is to analyze expression of CD160 in B-CLL and if there is relation with clinical and laboratory parameters.
The current study was carried out on thirty newly diagnosed CLL patients in addition to fifteen normal subjects who served as a control group.
Patients were clinically evaluated laying stress on the presence or absence of lymphadenopathy,splenomegaly and hepatomegaly, in addition to laboratory assessment .
Using flow cytometry, the studied cases were investigated for CD160 expression which was expressed as percentage . Surface CD160 was assessed where it found to be significantly increased in patients with CLL with high sensitivity and specificity .
This study demonstrated a highly significant increase in TLC, lymphocytic % and ALC and low Hb level and PLT count in patients compared to control group.
As regards organ infilteration the present study demonsterated precence of lymphoadenopathy ,splenomegaly and hepatomegaly among patients.
There was no significant statistical correlation between CD160 and Hb ,PLT ,TLC ,lymphocytic% ,ALC ,BML and LDH.
Also There was no significant statistical correlation between CD160 and CD23 ,CD79b , SmIg ,FMC7,CD5 and CD10.
But There was a significant statistical positive correlation between CD160 and CD20 and a highly significant statistical positive correlation
Between CD160 and CD19 and CD38.
There was no significant difference of CD160 expression in different stages in Binet and Rai staging
Conclusion
from this study we concluded that:
• CD160 was expressed on a greater percentage of B-CLL cells in patients than in control group with very high sensitivity and spcificity, so it can represent a useful diagnostic tool in B-CLL cases.
• CD160 expression may be usefule prognostic marker in patients with B- CLL.