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Abstract Il\YfRODUCTION Iron deficiency in calcareous or alkaline soils, is a worldwide problem in limiting production of crops including annual and perennial legumes. In Egypt, a high proportion of soil have a pH superior to 7.5 . In agroforestry, nitrogen fixing trees (NFT) are usually utilized for improving the mtrogen content of tropical soils and production of forage for animal feeds. (Galiana et al., 1990 and Sutherland & Sprent, 1993). Recent observation showed that some species ofleguminous trees seemed to have good growth in iron limited soils (Dr. Didier Lesueur, personal communication). Studies on siderophore - mediated iron uptake by rhizobia to improve nodulation and N2- fixing activity in legume - rhizobium symbiosis have attracted an increased number of investigators (Guerinot, 1991; Crowley et al., 1991; Barton et al., 1996). The establishing of a large collection of Rhizobium strains from NFT, isolated from calcareous or alkaline soils, is very important for increasing the N2- fixing potential of this symbiosis. The effect of siderophore production by rhizobia and other root associated microorganisms on growth, nodulation and iron uptake by leguminous hosts should also be evaluated. Therefore, this study was planned to examine the following aspects: 1- Possible relationship between adaptability ofN2-fixing tree species to Fe stress and siderophore production by root associated microorganisms 2- Qualitative and quantitative examination of siderophore production ability amongst rhizosphere microorganisms with special reference to rhizobia and fluorescent pseudo monads. 3- Evaluation of possible improvement of N2-fixing tree legume, sensitive to Fe stress, by using rhizobia effective in siderophore production. |