الفهرس 
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Abstract
In this study, sixty four samples were positive to Salmonella (23 isolates from broilers, 23 isolates from layers, 7 isolates from poultry feedstuffs, 2 isolates from chicken eggs and 6 from litter samples). Serotyping of isolated salmonellae were S. Typhimurium, S. Enteritidis, S. Kentucky, S.arizonae, S. Hydra, S. Anatum, S. Paratyphi A, S. Agona, S. Bloomsbury, S. Derby, S. Rubislaw, S.Senftenberg, S.Virchow and S.Cerro. Antimicrobial sensitivity test was performed. It was concluded that most of salmonellae were sensitive to Gentamycin, Amikacin, Tetracycline, Levofloxacin and Ceftriaxone in a percentage of 70.3%, 57.8%, 60.9%, 84.3% and 62.5%, respectively. Moreover, it showed moderate resistance to Tobramycin and Enrofloxacin in a percentage of 51.6% each. While, it showed high resistance to Ampicillin and Nalidixic acid with a percentage 84.4% and 68.6%, respectively. Most of isolates showed no resistance against Sulphamethaxole+Trimethoprim in a percentage of 89.1%. The study highlights that, SYBR Green real-time PCR test is a rapid, reproducible, and robust method for detecting Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Kentucky in poultry samples using primers SEFA-2 and SEFA-4 for S.Enteritidis, fli 15 and tym for S.Typhimurium and S.Kentucky respectively. The specificity of the reaction was confirmed by the Tm, which was consistently specific for the amplicon obtained; the mean peak Tm obtained with curves specific for serotype S. Enteritidis was 83.03°C while it was 84.04°C and 83.99°C for S.Typhimurium and S.Kentucky, respectively.