الفهرس 
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Abstract
SUMMARY
Aim of the work
his study is purposed to clarify the impact of maternal and
neonatal factors on total nucleated cell count of collected
umbilical cord blood of full term delivery > 36 weeks
completed gestation.
Methodology
The present study was conducted on a random cross
section of 50 full term deliveries at Maternity Hospital, Ain
Shams University. Deliveries were attended. The cord blood
was collected after paternal understanding and approval for the
procedure.
Comprehensive history taking was done for all stressing
on: Pre-existing maternal problems; fetal problem and natal
history.
Detailed clinical neonatal examination: Determination of
the gestational age using Ballard score, APGAR score at 1 & 5
minutes, determination of birth weight, length and occipitofrontal
circumference and cord and placental examination and
weight.
T
Summary
75
Results
This cross sectional study was conducted on 50 full term
newborns; 26 (54%) were males and 23 (46%) were females, with
GA of 38.34±1.117 weeks, birth weight 3347.0±355.196gm,
length 49.90±2.349cm, OFC was 35.20±1.399cm. The APGAR
score at 1 min ranged between 5-10 with median 8, while at 5 min
it ranged between 8-10 with median 9.
In our study maternal age was 27.68±1.52 years and
ranged between 25-32 years, mother weight ranged between
70-88 kg with mean±SD of 77.88±4.45 and mother Hb%
ranged between 11-13.5 % with mean±SD of 12.03±0.79.
Parity ranged between 1-8 live births with median of 3.
Placental weight was 742.20±103.633 gm and cord length was
59.50± 14.30cm. Thirty three neonates (66%) delivered
vaginally while 17 (34%) delivered by CS, 33 (66%) had no
anesthesia, 6 (12%) had spinal anesthesia and 11 (22%) had
general anesthesia.
Regarding resuscitation, ambu-bag and O2 were used in
10(20%) neonates while 40 (80%) neonates did not need them
during resuscitation.
Regarding way of collection 22 (44%) cord blood
samples were collected using Bag and 28 (56%) samples were
collected using syringe. No cord anomalies were detected in
this study.
Summary
76
Regarding maternal risk factors, 10 (20%) mothers had
hypertension while only 5 (5%) had preeclampsia, 7 (14%) had
PROM and all of them did not have gestational DM or fever
during that pregnancy.
Regarding neonatal blood groups, 24 (48%) were of
group O, 15 (30.0%) were group A, 6 (12.0%) were group B
and 5 (10%) were group AB. Regarding maternal blood groups;
29 (58.0%) were of group O, 8 (16.0%) were group A, 7
(14.0%) were group B and 6 (12%) were group AB. Regarding
Rh groups, neonatal RH was negative in 6 (12.0%) and positive
in 44 (88%), while maternal Rh was negative in 8 (16.0%) and
positive in 42 (84.0%).
There was significant higher TNC levels in neonates
delivered by CS compared to VD (p=0.012). No significant
difference in TNC levels regarding method of blood collection,
resuscitation or anesthesia.
There was statistically significant higher TNC in mothers
with hypertension and PROM (p=0.03, 0.001, 0.001
respectively). No significant difference in TNC regarding
parity, maternal blood group, RH or preeclampsia was detected.
TNC is positively correlated with GA, neonatal weight
and OFC. No significant correlation between TNC level and
neonatal length was detected.
Summary
77
A significant negative correlation between TNC and
maternal age and highly significant negative correlation with
maternal weight were detected. A significant positive
correlation between TNC and amount of cord blood was
detected. There was no correlation detected between maternal
Hb with TNC.
Highly significant positive correlation between TNC and
TLC and granulocytes were detected. No significant correlation
between TNC and other CBC parameters was detected.
Our logistic regression showed that after elimination of
all other factors, there was highly statistically significant
association between higher TNC and amount of cord blood,
TLC and granulocytes and a statistically significant association
between higher TNC and lower maternal weight.