الفهرس 
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Abstract
Subclinical mastitis is a persistent, inflammatory response of mammary tissue attributed to intramammary invasion of a mastitis-causing pathogen such as Streptococcus uberis (Rinaldi et al., 2010). Mastitis is usually considered the most costly disease of dairy cattle. where it affects the quality of milk and is a potential health risk for the other cows. Mastitis constitutes a source of economic loss for the dairy industry due to its effects on milk quality. It lowers the quality of cheese and other manufactured milk products (Politis and Ng-Kawai-Hang, 1988) and decreases milk yield. It also reduces nutritive value of milk due to the changes in its composition.
Due to the economic and public health importance of mastitis, the present study was designed to evaluate the use of new mastitis detection system in dairy cows and buffaloes in the term of sensitivity in comparison to well established used detection methods.
One hundred raw milk samples (50 cows and 50 buffalos quarter milk sample) were examined.
Milk somatic cell count, electrical conductivity, pH were tested for each sample in addition to interlukine-6 determination.
This study was designed to test a newly developed -advanced method that allows the highly specific detection of IL-6 (produced by macrophages and mediates acute systemic immune responses) in milk samples for detection of sub-clinical mastitis. The litter test was accomplished using ELISA specific for bovine IL-6 to quantify the IL-6 in whey of raw dairy cow and buffalo milk and to compare the new test’s result with an established method’ adopted in most dairy farms using SCC, milk electro-conductivity and pH.