الفهرس 
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Abstract
ABSTRACT
The present study was conducted to identify the variations
among clin
ical isolates of
Pasteurella multocida
at molecular level
to check the distribution of Pneumonia and hemorrhagic septicemia
in some farm animals sheep, buffalo and cattle at
Fayoum region in
Egypt
.
P. multocida
is one of the most important gram
-
negative
ba
cteria and is a commensal of the upper respiratory tract of many
animals. However, under predisposing circumstances the organism
is the etiological agent of a wide range of economically important
diseases. Thus to understand this microbial diversity,
chara
cterization of the isolates was done by their morphological,
biochemical and molecular characterization.
The five isolates chosen exhibited luxuriant growth on blood
agar with translucent grayish or yellowish green colonies.
However, they showed no growth
on MacConkey and no hemolysis
of blood, also the five isolates were positive for oxidase, catalase
and H
2
S
production, negative of urease and can ferment glucose,
fructose and glucose but can’t ferment lactose. Antibiotic sensitivity
showed that five isola
tes were sensitive to (tetracycline,
clindamycin and erythromycin) but resistant to (ampicillin,
trimethoprim/sulfamethoxazole and flucloxacillin). To determine
the genetic diversity nine random primers OPA
-
01
-
OPA09 were
used to/which generate different
profiles. The five strains of
p.
multocida
showed distinct RAPD profiles and relationships among
the five strains of
p. multocida
. The result of molecular
characterization the showed that five strains of
p. multocida
have
similar size of PCR products that
generated one band of 16S rDNA
and KMT1 gene that have 460 and ~1471 bp respectively. Thus
the
authors can recommend that diagnosis of
P. multocida
by combine
of 16S rDNA and KMT1 gene, antibiotic sensitivity should be
tested before curing of diseased anim
als. In order to reduce of use
antibiotic to devoid new resistant strains of
P. multocida.