الفهرس | Only 14 pages are availabe for public view |
Abstract It could be concluded from our findings that: (1) The formalized oil adjuvant bacterins prepared from locally isolated strains of M. gallisepticum and M. meleagridis were potent and induced considerable protecting antibody response and lowered the MG and MM population in the tracheae of turkeys. In this case we recommend the use of the formalized oil adjuvant vaccines prepared from locally isolated strains for protection of turkeys against Mycoplasma gallisepticum and Mycoplasma meleagridis infection. (2) SPA test is highly sensitive for detection of early formed antibodies against M. gallisepticum and M. meleagridis in sera of turkeys. (3) Enzyme-linked immunosorbent assay (ELISA) is recommended for evaluation of the immune response in vaccinated birds and it is the best serological test for detection of MG and MM antibodies in poultry sera as it has highest sensitivity, specificity and thus accuracy. (4) Polymerase chain reaction (PCR) is rapid, reproducible and sensitive technique for the identification of MG and MM. By this technique, we can avoid the false positive and false negative results encountered with the serological techniques. PCR overcomes the waste time and low recovery rates associated with cultural methods. |