الفهرس 
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Abstract
Biochemical and molecular studies on serotonin on relation to immune function were investigated.
One hundred adult male wistar albino rats of 6-8 weeks and average body weight 160-180 gm were used in the investigation of this study.
The animals were left 15 days before starting the experiment for acclimatization before the beginning of the experiment.
Experimental design:
Animals were randomly divided into 4 equal groups: each group contains 25 rats as follows.
1) Group 1 (control): Rats were subcutaneously administered with 0.5 ml of 0.9% saline solution.
2) Group 2 (serotonin group): Rats were subcutaneously administered with 0.5ml of Serotonin creatinin sulfate complex solution (100 mg/Kg body weight) on day 0.
3) Group 3 (serotonin+ sheep RBCs): Rats were subcutaneously administered with 0.5 ml of Serotonin creatinin sulfate complex solution (100 mg/Kg body weight) then after 30-60 minutes 0.5 ml of sheep RBCs (5%) was administered on day 0 by subcutaneous injection.
4) Group 4 (sheep RBCs): Rats were subcutaneously administrated with 0.5 ml of sheep RBCs (5 %) once on day 0.
Sampling:
Animals were sacrificed by cervical dislocation five times along the duration of the experiment after 2h., 1, 4, 7 and 11 days after