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العنوان
Molecular detection of antimicrobial resistance for some food borne pathogens /
المؤلف
Abd El-Salam, Azza Salah El Din.
هيئة الاعداد
باحث / Azza Salah El Din Abd El Salam
مشرف / Ahmed Mohamed Ammar
مشرف / Adel Attia Mohamed
مشرف / Mohamed Galal Aggour
الموضوع
Veterinary Bacteriology.
تاريخ النشر
2014.
عدد الصفحات
178 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Department of Bacteriology, Mycology and Immunology
الفهرس
Only 14 pages are availabe for public view

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Abstract

The widespread occurrence of multidrug resistance (MDR) in food borne bacterial pathogens is a global challenge and the problem is not limited to specific countries or bacterial pathogens.
Multidrug resistance is frequently linked with DNA elements such as integrons, which can disseminate genes encoding resistance to a number of antimicrobial drugs making a great impact on human health.
On the other hand, the glycopeptide vancomycin was considered to be the best alternative for the treatment of multidrug resistant MRSA. However, there are increasing numbers of reports indicating the emergence of vancomycin-resistant S. aureus (VRSA) strains in many intensive cares in different countries without full explanation for the sources of the dissemination of these strains. Thus, it is a significant issue to evaluate the incidence of VRSA among collected samples under study.
Therefore, the aim of the present study was to spot the light on some food borne microorganisms and antimicrobial resistant genes, which may transfer from meat products to human. Thus, it could be achieved through microbiological identification of the selected food pathogen, phenotypic characterization of antimicrobial resistance patterns of the recovered isolates, illustration the prevalence of vancomycin resistant S. aureus (VRSA) both phenotypically using MIC broth macrodilution method and genotypically by PCR, molecular characterization of class1 integron among multidrug resistant isolates and investigation of the integron gene cassettes by sequencing of PCR products to correlate resistance phenotypes with the presence of integron resistance genes.