الفهرس | Only 14 pages are availabe for public view |
Abstract The thesis comprises four main parts: Part I This part presents an introduction about the chemical name, structures, physical properties and pharmacological action of studied drugs. It also contains literature review for the reported methods of analysis of the selected drugs in pharmaceutical dosage forms as well as in biological fluids. Part II This part discusses the development and validation of an HPLC method for the determination of Cefotaxime. A kinetic investigation of the drug in different stress conditions was done. Also, it discusses the application of chemometrics to the handling of chromatographic response data. This was done following the kinetic degradation of CEF under three stress conditions: alkaline hydrolysis at three different temperatures 30, 50 and 70 ºC and acidic hydrolysis using two different concentrations of HCl (0.05 and 0.1 M) at four different temperatures 40, 60, 70 and 80 ºC. Derivative treatment (First and second) of chromatographic response data of CEF at the previously mentioned eleven cases followed by convolution of the resulting derivative curves using 8-points sin xi polynomials (Discrete Fourier functions) was done. This was found beneficial in improving linearity parameters (r, a, b, sy/x and F) of the response data. The application of Theil´s method, a non-parametric regression method, in handling the response data was compared with the least squares parametric regression method, which is considered the standard method used for regression. Theil´s method was found to be superior to the method of least squares in chromatographic response data. Finally, Arrhenius equation was applied on chromatographic response data after chemometric treatment as mentioned above for the parametric and non-parametric treatment of data in order to determine the half life (t1/2) of CEF in alkaline and acidic conditions at room temperature with high accuracy and it was found that both parametric and non-parametric treatment led to the same results. The estimated values of K25ºC and t1/2 at 25° C after the application of D2/FF method, which is assumed to correct for all types of errors, were in a good agreement with the reported values in literature. |