الفهرس | Only 14 pages are availabe for public view |
Abstract The main target of this study was to investigate the influence of feeding of some natural sources of coenzyme Q10 (soybean oil, grape seeds oil and broccoli) as compared to coenzyme Q10( CoQ10) on impaired liver function of rats injected with carbon tetrachloride (CCl4). Thirty five healthy adult male albino rats ”Sprague Dawley strain” weighing 150±15g , were used and divided into 7 equal groups, one was kept as a control (-ve) group, while the other groups were injected subcutaneously (s/c) by CCl4 in paraffin oil 50% v/v (2 ml/kg B.W.) twice a week for two weeks. The CoQ10 substitutes were given as a percent of 10% from the Basal diet but CoQ10 was given (1mg/kg B.W.). At the end of the experiment, feed intake (FI), body weight gain (BWG%), feed efficiency ratio (FER) and relative weights of liver, kidneys, spleen, lungs and heart were calculated. Also, proteins and some liver enzymes (SGOT, SGPT, ALP), total bilirubin, indirect bilirubin, direct bilirubin, antioxidant enzymes (SOD, GPx ,CAT), kidney function (Urea, CR, U.A), lipid profile (T.C, TG, LDL-C, VLDL-C and HDL-C) were determined in serum. Moreover, the liver , kidney and heart were histopathologically examined. The results indicated that rats injected with CCl4 recorded significant changes levels of all above biological and biochemical parameters. Histopathological examination supported these findings and revealed a marked lesions . Supplementation with natural sources of CoQ10 such as soybean oil, grape seeds oil and broccoli, in diets of hepatointoxicated rats led to a significant improvements in the activity of liver enzymes, antioxidant enzymes, kidney functions and lipid profile. It could be concluded that the phytogenic natural sources of CoQ10 were effective in protecting against CCl4-induced hepatointoxication liver functions and its side effects . Thus, this natural sources could be used in future as nutraceutical for hepatointoxication of humans. |