الفهرس 
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Abstract
Foot and mouth disease is one of the most important viral diseases affecting cattle, buffaloes and cloven-hoofed animals. It causes severe economic losses due to high morbidity, loss in meat and milk yields and their secondary complications.
So the disease becomes in the spotlight of the international and local levels in many countries to control and prevent its spread depending on early diagnosis of the disease, vaccination and strict quarantine measures in addition to good animal care.
The causative agent of FMD is a picornavirus of the genus Aphthovirus belonging to family Picornaviridae. The FMDV transmitted via inhalation, ingestion, and direct contact with infected animals.
FMD is prevalent in many parts of Australia, Asia, Europe and Africa, including Egypt.
Diagnosis of Foot and mouth disease depends on isolation and serological detection of virus antigen. Therefore the current study aims to conduct a Serological survey in addition to isolation and identification of the virus in cattle and buffaloes at different areas in Menofeia governorate.
The Applied tests have shown the following:
1- By using serum neutralization test for detection of FMDV serotypes O and A antibodies in cattle and buffaloes sera, a Serological examination of 230 serum samples obtained from 134 cattle and 96 buffaloes at different localities in Menofeia governorate.
It was found that from 146 examined sera 99 (67.8%) in cattle were