الفهرس 
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Abstract
In the present study, we examined the spatiotemporal expression of MPP6 (membrane protein palmitoylated 6) and its relation with CADM1 (cell adhesion molecule 1) in the mouse testis by Western blotting and immunohistochemistry. MPP6 immunoreactivity was demonstrated in the cytoplasm of step 11 to 16 spermatids from 5 weeks postpartum through adulthood. Double immunofluorescence microscopy demonstrated that MPP6 and CADM1 were colocalized only in the elongated spermatid. Furthermore, the immunoblotting of MPP6 in the testis of CADM1-deficient mouse showed a significant reduction to approximately 50% than that of wild-type mouse testis. In the testis of CADM1-deficient mice, the number of MPP6 immunopositive elongated spermatids decreased and the immunoreactivity for MPP6 was detected with dot-like or granular pattern in the cytoplasm of elongated spermatids. On the other hand, both MPP6 and CADM1 were normally expressed in the testis of Nectin-2 and -3 deficient mice referring that the involvement of MPP6 in spermatogenesis is specific for CADM1. These findings suggest that there is a strong relation between CADM1 and MPP6 and the interaction between both molecules has an essential role in spermatogenesis.