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العنوان
Bacteriological Studies on the transmission of Helicobacter pylori /
المؤلف
Hagger, Nehal Ibrahim Abdel- Fattah Ibrahim.
هيئة الاعداد
باحث / Nehal Ibrahim Abdel- Fattah Ibrahim Hagger
مشرف / Mahmoud A. Sweilm
مناقش / Fathy A. Mansour
مناقش / Nahed M. Ayyat
الموضوع
Helicobacter pylori. Botany.
تاريخ النشر
2013.
عدد الصفحات
88 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة بنها - كلية العلوم - Botany
الفهرس
Only 14 pages are availabe for public view

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Abstract

The peptic ulcers are now considered as infectious disease and the role of H. pylori in gastric cancers and other diseases of the upper gastrointestinal tract is being recognised or is being actively evaluated. H. pylori is a small microaerophilic, non-sporing, gram-negative bacteria. It is like curved rods, 3.5 ¼m long and 0.5- 1 ¼m wide, with multiple unipolar-sheathed flagella. It has a spiral shape in young cultures and can assume a coccoid form in older cultures. The prevalence of H.pylori in developing countries is approximately 100% and 50% in developed countries. 1- Isolation of of H. pylori in biopsies there were taken by a specialist from 50 patients (16 males and 34 females) complaining from gastroduodenal disorders with ages ranging from 25 -75 years. Samples taken were 35 sputum samples, 29 dental plaque, and 35 stool samples. The isolation occurred on chocolate agar media. 2- Identification of H. pylori by gram stain, urease, catalase, and oxidase tests. 3- Studying H. pylori transmission from food stuffs, water, and milk by taking 20 water samples (10 before station, 10 after station), 10 milk, 10 luncheon, 10 frankfurt, 10 sausage, and 5 beef samples from enviroments surrounding patients. 4- The antibiotics sensitivity test of identified culture of H. pylori was done. This test indicated that the organism was sensitive to Cefotaxime , and Ertapenem ), followed by Ceftriaxone, Imipenem, and Ceftazidime, while H.pylori showed less sensitivity to Levofloxacin, Ciprofloxacin, and E-Moxaclav giving rise to zone diameters, respectively H. pylori was high resistant to the other six examined antibiotics. The use of accurate biochemical methods for identification of H. pylori may eventually improve anti-H. pylori therapy. 5- Plant extract sensitivity test show that H.pylori is highly sensitive to white pepper, Cinnamomum zeylanicum, Syzygium aromaticum, and followed by black pepper, Apium graveolens, Cuminum cyminum . H.pylori showed less sensitivity to Martricaria chamomilla, Elettaria cardamomum, Solenostemna arghel, and highly resistant to Nigella sativa, Foeniculum vulgare, Zinigber officinalis, Rosmarinus officinalis, Allium sativum. The usage of medicinal plants is safe. 6- The prevalence of H. pylori in females is higher than males. 7- Helicobacter pylori was absent in all samples of dental plaque , and with higher prevalence in stool samples than sputum samples. 8- Younger ages have the high percent of H. pylori infection. ( representing the increase in the rates of pollution). 9- Some foodstuffs has the role in the transmission of H. pylori especially sausage, luncheon ,and frankfurt. 10- Water samples proved that there is no significant correlation between water and H.pylori prevalence.