الفهرس 
Families of cellulasesOnly 14 pages are availabe for public view
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Abstract
The present experiments were carried out at the microbial biotechnology department and other labs in Genetic Engineering and Biotechnology Research Institute, Sadat city, Sadat University, Egypt.
The main objective of the present investigation was the production purification and characterization of microbial cellulose hydrolyzing enzyme. This study was carried out as following :-
1) Isolate Bacteria that hydrolyzed the cellulose based polysaccharide.
2) Purification and characterization of Bacterial isolates.
3) Purification and characterization cellulose hydrolyzing enzyme.
The first experiment was the screening for cellulosic activity of Bacteria isolated from soils at ”El-Bagour city”. The result was 25 positive isolates recognized that hydrolysis of CMC and three isolates marked An5, An17 and An19 were found to be the most potent isolates.
The second experiment was the cloning and sequencing of cellulase encoding gene.The Third experiment was the production and purification of cellulose hydrolyzing enzyme using salting out fractions, gel filtration and ultra filtration.