الفهرس 
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Abstract
In the present study whispovirus immediate early 1 promoter (ie-1) was used to initiate surface expression of hemagglutinin (HA) of of Egyptian H5N1 Avian influenza virus (AIV). (HA) gene and whispovirus ie-1 promoter sequence was synthesized as expression cassette (ie1-HA) and cloned into pFastBac-1 transfer vector of Bac-To-Bac baculovirus expression system then transformed into DH10Bac competent cells for generation of recombinant bacmid via site specific transposition, the recombinant bacmid was used for transfection of Spodoptera frugiperda (Sf9) insect cells for construction of recombinant virus and expression of HA protein of H5N1 AIV. The recombinant glycoprotein expressed in Sf-9 cells showed hemadsorption activity, Hemagglutinating (HA) activity was also detected in both extra- and intra-cellular recombinant HA. Both HA and hemadsorption activities were inhibited by polyclonal anti-H5 sera. Significant expression of the recombinant protein was observed on the surface of infected insect cells demonstrated by Immunofluorescence. SDS-PAGE of the expressed analysis revealed the presence of visually distinguishable band ~63kDa in size that was absent in the non-infected cell samples. Western blot analysis confirm that the distinct 63 kDa band is corresponding to the recombinant HA glycoprotein of H5N1 AIV. The study reports the successful expression of HA protein of H5N1 AIV .The expressed protein was displayed on the plasma membrane of infected insect cells under control of whispovirus ie-1 promoter using baculovirus expression vector system.