الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 128 |  |  |
| | | from | 128 | | |
Abstract
This study aimed to evaluate the biological function of infant foods
fortified with lactoferrin and find out the effect of lactoferrin on some of
physiological parameters in healthy rats such as blood glucose, relative
weight of the organs, blood lipids, liver and kidney functions, serum Ca and
P .
The name lactoferrin is derived from its past classification as a major
iron-binding protein in milk. Lactoferrin, also referred to as lactotransferrin,
was first identified in 1939 in bovine milk, and in 1960 it was isolated from
human milk. Subsequently it has also been shown to be a major iron-binding
protein of other exocrine secretions such as bile, pancreatic juice and small
intestinal secretions, and has been localized in a host of other tissues, both in
man and in other mammals.The size and structure of lactoferrin is closely
related to that of another group of iron-binding proteins, the tranferrins, and
lactoferrin is considered by many to be a member of the transferrin family.
Lactoferrin is a glycorptein with a molecular weight of about 80 kDa, which
shows high affinity for iron. The molecular structure and amino acid
sequence of human lactoferrin were discovered in 1984.
A total of 24 growing male albino rats (Sprague Dawley strains) of an
average weight (100‐110) g. and age (2 months) Divided into 4 groups, 6
rats in each group and fed several diets for 28 days . Each rat was housed in
special cage under controlled condition.
96
Summary
Every day the animals were observed for the external appearance,
shape, color , distribution of hair and physical activity. All rats were fed for
3 days on the basal diet before the beginning of the experiment. The rats
were weighed aer 3 days (each separately) aer that weighed once a
week for 4 weeks. The diet was presented to rats in special covered cups to
avoid food loss. All rats were provided with water by glass tubes through
wire cage. New food was given according to the actual need of each group.
The final weight was recorded and the organ’s weight were calculated.
Aer feeding on basel diet for 3 days (adaptaon period). Six
rats were kept as control wich fed basel diet during the experiment
period G1(normal control).The other groups were fed several diets as
follows:
Group 1: (normal control group):was fed Basal diet only.
Group 2: was fed diet consists of (Basal diet +0.1 %.lactoferrin).
Group 3: was fed diet consists of (Basal diet + 0.2 % lactoferrin).
Group 4: was fed diet consists of (Basal diet + 0.3% lactoferrin).
At the end of the experiment. rats were fasted overnight and
anesthetized, blood samples were collected in dry centrifuge tube from
hepatic portal vein. The organs (liver, kidney,heart,lungs and spleen) were
removed, washed with saline and dried by filter paper then weighed .
Data revealed the follwing lactoferrin effects on investigated indices:
97
Summary
• With lactoferrin supplementation, body weight gain and food intake ,
values showed non significant differences at 0.1% and 0.2%
concentration, and significant difference at 0.3% concentration.
• Rats fed expermintal diets showed a gradual increase of relative kidney
weight.
• Rats fed expermintal diets showed a gradual decrease of relative heart
weight.
• With lactoferrin supplementation relative weight of organs (spleen and
lungs), values showed non-significant effect.
• With lactoferrin supplementation relative weight of organs (liver), values
showed slight but non-significant difference at 0.1%and 0.2%
concentration and significant difference at 0.3% concentration.
• Data illustrated that rats fed lactoferrin, showed lower values for blood
glucose.
• Rats fed experimental diet showed a gradual decrease of (AST,ALT and
ALP).
• Rats fed experimental diet showed that serum albumin had nonsignificant
increase in 0.2% and 0.3% of lactoferrin groups. And serum
albumin had non-significant decrease at 0.1% of lactoferrin.
• With lactoferrin supplementation, serum creatinine, values showed non
significant difference at 0.2% and 3% concentration, and significant
differences at 0.1% concentration.
98
Summary
• With lactoferrin supplementation, serum urea, values showed significant
difference at 0.1% and 3% concentration, and non significant differences
at 0.2% concentration.
• Rats fed experimental diets showed a gradual decrease of serum uric acid
level.
• With lactoferrin supplementation, total cholesterol, triglycerides, VLDLcholesterol
and LDL-cholesterol, values showed non- significant
differences at 0.2% concentration. And significant differences at 0.3%
concentration.
• With lactoferrin supplementation, HDL-cholesterol, values showed nonsignificant
increase effect at 0.1% and 0.2% concentration. And
significant increasing at 0.3% concentration.
• With lactoferrin supplementation serum Ca, values showed nonsignificant
differences at 0.1% and 0.2% concentration and significant
difference at 0.3% concentration.
• With lactoferrin supplementation serum P levels Improved significantly.