الفهرس 
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Abstract
In the present study Sixty eight adult female Wistar rats (200–250g body weight) were housed in cages under a temperature-controlled environment of 24 ± 1 ° C with a 12-hour dark/light cycle (dark cycle: 8: 00 p.m. to 8: 00 a.m.) and housed with free access of food and water throughout the experiments. The animals were divided into four groups used for behavioral and histological studies. (1). Normal control group: age-matched rats (n=16) received no manipulations, served as normal controls. (2). Lesioned group: rats (n=16) spinal cord was destructed through needle approach. (3). Sham lesioned control group: rats (n=16) were given injection of vehicle (4μl of CSF) in the spinal cord. (4). Adult human olfactory bulb derived neural stem cell (OBNSCs) transplantation group (treated group): rats (n=20) received OBNSCs transplantation 7 days after spinal cord lesion
The different types of staining methods histopathological findings, immunohistochemistry and immunocytochemistry were used to study the morphological features of spinal cord and the ability of our hNGF-GFP-OBNSCs for in vitro and in vivo proliferation and differentiation into different types of neuronal and glial (astrocytes, oligodendrocytes) elements had encouraged us to assesses the possible therapeutic potential of hNGF-GFP-OBNSCs for SCI at the pre-clinical level using SCI rat model
Conclusion: It could be concluded that adult hNGF-GFP-OBNSCs has the ability to proliferation, migration and differentiation but without restoring normal furcation after spinal cord injury due to hostile environment of spinal cord after injury.