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العنوان
Seriological studies on the different antigens of avian salmonellae /
المؤلف
Abd El-Rahman, Medhat Ahmed Sadek.
هيئة الاعداد
باحث / مدحت أحمد صادق عبد الرحمن
مشرف / على محمد سليمان الجد
مناقش / أحمد أحمد على البسيونى
مناقش / أشرف عواد عبد التواب
الموضوع
Bird watching.
تاريخ النشر
2005.
عدد الصفحات
215 p. ;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Food Animals
تاريخ الإجازة
1/1/2005
مكان الإجازة
جامعة بنها - كلية الطب البيطري - Bacteriology, immulogy and mycology
الفهرس
Only 14 pages are availabe for public view

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Abstract

Salmonella infections are considered to be the most important disease affecting ducks, geese, turkeys and chickens. The disease causes severe damage among young birds with a high mortality rate. Adult birds are often chronic carriers of salmonella organisms without outward signs. So, this study was planned to follow up the disease through detection of salmonella carrier birds, isolation of the organisms from these birds and evaluation of different salmonella antigens by different serological tests:
1.Salmonella organisms were isolated from different poultry farms using (cloacal swabs, floor litter, drag swabs) with total isolation of: 2.8% (turkey farms), 4.17% (duck farm), 4.4% (chicken farm) and 2.6% geese farm.
2.About 162 isolates of salmonella were identified (48 isolates from ducks, 30 isolates from geese, 51 isolates from chickens and 33 isolates from turkeys) and the isolates were serotypes as: S. typhimurium, S. enteritidis, S. virchow, S. pullorum, S. meleagridis, S. infantis, S. anatum, S. montevideo and S. heidelberg.
Summary
3.Different salmonella antigens were made from the 5 strains (S. typhimurium, S. enteritidis, S. virchow, S. pullorum, S. meleagridis) which representing group B, C, D, E of salmonellae.
4.Serological survey for detection of salmonella carriers by the rapid whole blood agglutination test using the polyvalent stained salmonella antigen. Out of 4000 birds, the incidence ratios were: 9% for ducks, 10.5% for geese, 16.4% for chicken and 7.5% for turkey.
5.Comparing the efficiency of different stained monovalent and polyvalent antigen for testing 2000 birds (500 of each of ducks, geese, turkeys and chickens). The polyvalent antigen detected the highest positivity as: 7.4% (geese), 12.2% (turkeys), 10.4% (ducks) and 9% (chickens).
6.It was clear from experimental study after oral inoculation of ducks, geese, turkeys and chickens with S. enteritidis, S. meleagridis, S. typhimurium, S. pullorum and S. virchow, the following results were obtained: by using the different agglutination test (Rapid whole blood agglutination test, rapid serum agglutination test, tube agglutination test, micro-agglutination test and flagellar tube agglutination test):
a. Serological testing of turkeys experimentally infected by S.
meleagridis, S. typhimurium, S. virchow, S. enteritidis and S. pullorum recorded the highest positivity by tube agglutination and micro-agglutination as: 90%, 70%, 60%, 60% and 77.7% respectively.
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Summary
b.Serological testing of chickens experimentally infected by S. meleagridis, S. typhimurium, S. virchow, S. enteritidis and S. pullorum recorded that highest positivity by the tube and micro-agglutination test as: 70%, 70%, 60%, 90% and 90% respectively.
c.Serological testing of ducks experimentally infected by S. meleagridis, S. typhimurium, S. virchow, S. enteritidis and recorded the highest positivity by the tube and micro-agglutination as 60%, 88.8%, 70%, 100% and 66.6% (S. pullorum) by rapid serum agglutination test respectively.
d.Serological testing of geese experimentally infected by S. meleagridis, S. typhimurium, S. virchow, S. enteritidis and S. pullorum recorded the highest positivity by tube agglutination and micro-agglutination as: 70%, 70%, 60%, 70% and 70%.
Concerning the incidence of positive reactors as tested by ELISA technique using the three subcellular antigens (lipopolysaccharide, sonicated antigen and flagellar antigen). The following results were obtained:
a.The highest positive reaction of antibodies induced by S. enteritidis experimentally infected chickens was 100% during 4-7 weeks post infection by the use of lipopolysaccharide antigen.
b.Lipopolysaccharide antigen detected 90% of chickens experimentally infected by S. pullorum on the 4th week post infection.