الفهرس 
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Abstract
Cystic echinococcosis (CE) is one of the most important parasitic zoonotic diseases which cause a public health and economic problem all over the world. A survey on CE in camel and sheep slaughtered at El-Basatin abattoir, Cairo, Egypt, was conducted during the period from January 2012 to December 2012. The incidence of CE was 7.5% and 0.96% among examined camel and sheep. Out of 33 infected camels 87.9% and 12.12% were found to have the infection in their lungs and liver respectively. While in sheep infection was only restricted to the liver. In camel, 26.7 %, 45.6 % and 27.8 % of pulmonary cysts and 25.0 %, 25.0 % and 50.0 % of hepatic cysts were small, medium and large sized cysts respectively. Collected cysts of sheep were small and medium in size. Out of 94 examined cysts 66 (70.2%) were fertile and viable and 3 (3.2%) were fertile but not viable, 9 (9.6 %) were sterile and 16 (17.0 %) degenerated respectively. Camel lung had 67 (74.4 %) fertile, while in the liver 2 (50.0 %) were fertile. The 2 cysts found in sheep liver were caseated. Biological studies were conducted through experimental infection of rabbits with Hydatid cyst protoscolices (PSC) of camel origin. Rabbits examination 13 w.p.i. revealed the presence of small Hydatid cysts (HC) (0.5- 12 mm in size) in 2/4 of rabbits distributed in organs of peritoneal cavity except from liver, while chest cavities were free from infection. The sensitivity and specificity of ELISA using purified HC fluid antigen (HCF) of camel origin were determined as 75% and 68.75% in camel and 100% and 38.09% in sheep. While the sensitivity and specificity of ELISA using PSC antigen of camel origin were determined as 100 % and 46.87 % in camel and 100 % and 21.95 % in sheep. While using ELISA in rabbit cleared appearance of antibody levels at 2 w.p.i and increased gradually till 10 w.p.i and nearly remained in a constant level till the end of the experiment. The SDS-PAGE analysis of camel purified HCF antigen indicated that 7 specific-protein bands were detected at molecular weight 174.3, 99.0, 70.0, 65.6, 48.0, 26.0 and 18.5 KDa. While the PSC antigen showed 4 specific protein bands at molecular weight 181.8, 92.5, 62.3 and 16.6 KDa. ELTB analysis showed presence of 4 protein bands corresponding to molecular weight standard at 22, 26, 31 and 51 KDa react specifically against sera infected with HC. While 48 and 65 KDa protein bands found to be non specific bands as they showed positive reaction in both HC positive sera and sheep sera infected with C.ovis. Also 48 KDa protein bands gave positive reaction when tested against sheep sera infected with C.tenuicollis and negative control sera of rabbit.