الفهرس | Only 14 pages are availabe for public view |
Abstract Six Newcastle disease virus (NDV) field strains (NDV-QF10, NDV-QF13, NDV-QH10-1, NDV-QH10-2, NDV-QQ09 and NDV-QP09) were isolated and pathotyped from samples collected in Qatar from captivated falcons, houbara bustard, quail and pigeons, respectively, during the study period of 2012 and 2013. Virus cultivation in specific pathogen free embryonating chicken eggs (SPF ECE), haemagglutination (HA), haemagglutination inhibition (HI) and mean death time (MDT) assays were used in the investigation to reveal virus identity and pathogenicity. Real time reverse transcriptase polymerase chain reaction (rRT-PCR) was embloyed as a confirmatory technique and it successfully detected NDV cDNA fragments amplified from RNA extracts of amnioallantoic fluids (AAF) of the inoculated SPF ECE. The six NDV isolates were classified velogenic strains. Sequence analysis and phylogenesis of the corresponding cDNA revealed 112R-R-Q-K-R-F117 amino acid motif confirming velogenicity of three of the six strains, and that all are belonging to NDV genotype group VII. |