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Abstract A total of 595 random samples including (swabs, muscles and skin) were taken from seventy broiler carcasses, via different processing stages and finished product, collected from two processing plants of different levels of hygienes mainly plant I (automatic system) and plant II(manual or prmitive system). All samples were subjected to bacteriological examination. The obtained results revealed the following: Isolation and count of S. aureus from processing stages and finished product: 1- The incidence of S.aureus isolated from swabs of vent and skin flap of neck taken from plant I was (34.28%), (51.42%), and (51.42%), (57.14%), from live birds and slaughtered chicken, respectively. 2- The incidence of8.aureus isolated from swabs of vent and skin flap of neck takeu from plant II was (40010),(57.14%) and (51.42%), (62.85%) from live birds and slaughtered chicken, respectively. 3- The incidence of 8.aureus isolated from processing stages of plant I which conItituted (SA Def), (SAW.) and (SACh) was (62.85%), (62.15%) and (68.57%), respectively. 4- The incidence of S.aureus isolated from processing stages of plant IT whida constituted (S.A. Det) and (S.A.W) was (68.57%) in both. the chilling stage was not envolved in plant IT. 5- The incidence of S.aureus isolated from skin and muscles of finished product in plant I was (68.57%) and (45.71%), respectively. 6- The incidence of S.aureus isolated from skin and muscles of finished product in plant II was (85.71%) and (74.28%), respectively. 7- The log means of total S.aureus count /cm2 of the examined broiler swab samples taken from (A. Def), (A.W) and (A.ch) stages were (2.395), (2.012) and (2.313), respectively. 8- The log means of total Siaureus count /cm2 of the examined broiler swab samples taken from (A. Det), (A.W) stages in plant II were (2.933) and (2.451), respectively. 9- The log means of total S.aureus count from examined broiler samples (skin/em’ and muscle/g) of plant I were (2.579)and (1.798), respectively. 10- The log means of total S.aureus count from examined broiler samples (skin/cm2, and muscle/g) of plant II were (3.875%) and (2.489), respectively. Biotyping of the isolated strains from proscessing stages and finished product. Enterotoxigenicity of isolated S.aureus strains from finished product revealed that. A- Twenty-four strains of S.aureus isolated from finished product from plant I were examined for enterotoxigenicity and 13 (54.16%) of them were positive for toxin production and identified as follows: 2 strains type (A) , 4 (C), 2 (D) as single producer and one strain from each of(A+B), (A+C), (A+D), (B+C) and (A+C+D) as multiple produces. B- Thirty strains of S.aureus isolated from fmished product from plant II were examined for enterotoxigenicity and 26 (86.7%) of them were positive for toxin production and identified as follows: S strains type (A) 4 type (B) one strain type (C) and 8 strains type D as single producer strains and 3 strain produced (A+C+D) and 2 strains produced (B+C) beside 3 strains produced (A+B+D). (C+D), (A+D). Enterotoxins A and D were completely inactivated after autoclaving (30 minutes) and roasting (30 minutes) while boiling (30 minutes) had no effect on the stability of Enterotoxins and fiying had a little effect. |