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Abstract Protozoa seemed to exhibit a well defined condition as a major world food producing animal health problem. A total of 300 blood samples and 2632 samples of internal organs and muscles collected from 329 animals (70 buffaloes, 52 cattle, 59 camels, 46 sheep, 52 goats and 50 calves) slaughtered at Kalubia abattoirs. were examined for presence of protozoa moreover, a total of 64 faecal samples were collected from stray cats at abattoirs in question were also examined. Macroscopic examination revealed that SaI”cocystisfusiformis was only found in examined oesophageal samples of buffaloe (40%) and cattle (38%). Microscopic Sarcocystis were not detected by impression smears in all examined samples of different species. The use of digestion technique clar’ified that regardless of examined organs, cattle constitltted the highest animals species infested with Sarcocystis (78.85%) while buffaloe represented the lowest infested animal species (62.86%). No Sarcocvstis were detected in all the examined samples collected from calves. The obtained results revealed that the highest infested samples of the examined buffaloes, cattle and camels with Sarcocystis were those taken from oesophagus (51.43% , 65.38% and 42.47% , respectively). Moreover, the examined heart samples for sheep (69.57%) and muscles of fore quarter for goats (75%) were the highest infested samples with Sarcocystis. No Sarcocystis were detected in the examined samples collected from lung of cattle, liver of camels and goats. All the examined samples of brain were free from Sm-cocyst is . Histopathological examination showed that all the examined samples of brain, lung Liver were free from microscopic Sarcocystis. Sarcocystis were detected in all examined samples of heart, diaphragm, oesophagus and muscles of fore quarter of slaughtered animals at varying percentages. Toxoplasma organisms were not detected in all the examined samples of different animals by impression smears, digestion technique or histopathologically. Prevalence of Toxoplasma Oocysts among examined cats was 18.75% • The highest incidence of seroposlt ive against Toxoplasma antibodies by using dye test was observed in sheep (42%) followed by goats (34%), buffaloes (28%) and camels (10%), while all the examined sera of cattle and calves were seronegative against Toxoplasma antibodies. The incidence of titers (’14 , ’/16 and I,..) were given. Distribution of titers showed that the sum of low and mild titers was greated than the moderate titers. Toxoplasma cysts could survive freezing at -20 oC for 2 hr, therefore, cats fed on that meat could shed Oocysts in their faeces. All infectious stages of Toxoplasma were rendered non infective by exposure to a temperature of 40 ”C for 1.5 hr, 6O”C for 5 min or 70 ”C for 2 min. Importance of protozoa as well as suggested hygienic measures to control toxoplasmosis were discussed. |