الفهرس 
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Abstract
Till now the incidence of equine arteritis virus in Egypt is still unknown and need more investigations. This initiated the present design of a protocol for an isolation trials and characterization of equine arteritis virus from field specimens. A total of 548 samples were collected from governmental and private studs of Arabian horses and also foreign horse breeds, including 540 serum samples, 4 EDTA-blood, 4 semen samples. Serologically, indirect ELISA were performed on 540 serum samples. 130 samples were clearly strong positive by percentage 24%. Molecular detection of EAV genome was performed using real time RT-PCR only on 8 selected semen and EDTA-Blood samples from highly distinct positive ones by indirect ELISA. All samples were found negative to presence of EAV genome by real time RT-PCR. Trials of isolation on different types of cell cultures were done on the 4 described semen samples, RK-13, VERO-1008, BHK-21 AND MDBK cell lines were used in this trials. six blind serial passages were done using the described samples; all samples were found negative for the presence of CPE for EAV, the sixth passage of all samples on RK-13 cells re-tested with rRT-PCR to confirm absence of EAV and the result was negative to presence of EAV genome which assure absence of EAV in collecting areas of clinical specimens.