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العنوان
Polymerase Chain Reaction For Detection Of Salmonella Enteritidis In Chicken carcasses /
المؤلف
El-Gammal, Ghada Ahmed Mohamed.
هيئة الاعداد
باحث / غادة احمد محمد الجمال
مشرف / حلمى احمد السيد تركى
مناقش / محمد محمد موسى
مناقش / طلعت حامد شعيشع
الموضوع
Chiken.
تاريخ النشر
2005.
عدد الصفحات
118 p. ;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
التمريض
تاريخ الإجازة
1/1/2005
مكان الإجازة
جامعة بنها - كلية الطب البيطري - Department of Microbiology
الفهرس
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Abstract

This work was carried out to survey Salmonella infection in
broiler chicken carcasses at small poultry processing shops in Kafr
El-Sheikh province. The comparison of ordinary method for
bacteriological examination and delayed secondary enrichment
(OSE) procedures, as well as the effect of chemotherapeutic agents
on Salmonella isolates, in addition to detection of Sal. Enteritidis
by using polymerase chain reaction (PCR).
The results are summarized in the following:
• Out of 152 examined broiler chicken carcasses, 8 were positive
for Salmonella at a percentage of 5.26%.
• On comparison between ordinary method of isolation and OSE,
3 Salmonellae isolates were recovered using ordinary method
while 8 isolates were recovered using OSE.
• Using PCR for detection of the serotype of Sal. Enteritidis, 7
isolates from the 8 isolates were Sal. Enteritidis representing
4.6% of the total 15a examined samples and 87.5% from the 8
isolated Salmonellae;
• The in vitro sensiti~ test revealed that ciprofloxacin gave the
highest activity agailllst Salmonella isolates (87.5%) followed by
chloramphenicol, etf’ofloxacin and norfloxacin (75% for each).
On the other hand,I.n 8 Salmonella isolates were resistant to
amoxycillin, penicilliP and spectinomycin (100%).